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Bone biology‐related gingival transcriptome in ageing and periodontitis in non‐human primates
Author(s) -
Pandruvada Subramanya N.,
Gonzalez Octavio A.,
Kirakodu Sreenatha,
Gudhimella Sudha,
Stromberg Arnold J.,
Ebersole Jeffrey L.,
Orraca Luis,
GonzalezMartinez Janis,
Novak Michael J.,
Huja Sarandeep S.
Publication year - 2016
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12528
Subject(s) - transcriptome , periodontitis , ageing , biology , dental alveolus , bone resorption , microarray , connective tissue , periodontium , gene expression , periodontal fiber , microarray analysis techniques , medicine , gene , dentistry , genetics
Abstract Aim Cellular and molecular immunoinflammatory changes in gingival tissues drive alveolar bone loss in periodontitis. Since ageing is a risk factor for periodontitis, we sought to identify age‐related gingival transcriptome changes associated with bone metabolism in both healthy and in naturally occurring periodontitis. Materials and Methods Adult (12–16 years) and aged (18–23 years) non‐human primates ( M. mulatta ) ( n = 24) were grouped into healthy and periodontitis. Gingival tissue samples were obtained and subjected to microarray analysis using the Gene Chip Macaque Genome Array. Gene expression profiles involved in osteoclast/osteoblast proliferation, adhesion and function were evaluated and compared across and between the age groups. QPCR was also performed on selected genes to validate microarray data. Results Healthy aged tissues showed a gene profile expression that suggest enhancement of osteoclastic adhesion, proliferation/survival and function ( SPP 1, TLR 4, MMP 8 and TFEC ) and impaired osteoblastic activity ( SMEK 3P and SMAD 5). The gingival transcriptome in both adult and aged animals with naturally occurring periodontitis ( FOS , IL 6, TLR 4, MMP 9, MMP 10 and SPP 1 genes) was consistent with a local inflammatory response driving towards bone/connective tissue destruction. Conclusion A pro‐osteoclastogenic gingival transcriptome is associated with periodontitis irrespective of age; however; a greater bone‐destructive molecular environment is associated with ageing in healthy tissues.