Premium
The use of endothelial progenitor cells combined with barrier membrane for the reconstruction of peri‐implant osseous defects: an animal experimental study
Author(s) -
Machtei Eli E.,
Kim David M.,
Karimbux Nadeem,
ZigdonGiladi Hadar
Publication year - 2016
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12511
Subject(s) - osseointegration , dentistry , medicine , implant , buccal administration , progenitor cell , urology , ligature , peri , granulation tissue , surgery , stem cell , wound healing , biology , genetics
Background The aim of this research was to study the efficacy of endothelial progenitor cells ( EPC ) with ß tri‐calcium phosphate (β‐ TCP ) and barrier in the treatment of experimental peri‐implantitis. Methods Thirty implants were placed in five American foxhounds. Following osseointegration, ligature‐induced peri‐implantitis was initiated. EPC were isolated from peripheral blood. Two months later, open flap debridement was performed and implants’ surface was decontaminated with 24% EDTA . The sites were grafted with β‐ TCP and collagen membrane (β‐ TCP group) or β‐ TCP loaded with EPC and covered with membrane ( EPC group) or left un‐grafted ( OFD group). At 3 months, animals were killed and specimens sent for histomorphometric and micro‐ CT analysis. Bone height and % BIC served as primary and secondary outcomes. Results EPC group showed shorter distance to first BIC (3.29 ± 0.69 mm) compared to 4.2 ± 0.92 mm (β‐ TCP ) and 3.82 ± 0.73 mm ( OFD ). Mean histological BIC was 2–3 folds higher in the EPC group (17.65 ± 3.3%) compared with OFD (7.55 ± 2.24%, p = 0.01) and β‐ TCP (5.68 ± 2.91%, p = 0.05). BIC greater than 25% was found only in the EPC group. The distance between the implant shoulder to 1st BIC was 2.51 ± 0.7 mm (lingual sites) compared to 3.64 ± 0.8 mm for buccal sites ( p < 0.0001). Conclusion β‐ TCP loaded with EPC and covered with a membrane‐enhanced bone formation around previously contaminated dental implants in an experimental peri‐implantitis canine model.