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Stem cells derived from “inflamed” and healthy periodontal ligament tissues and their sheet functionalities: a patient‐matched comparison
Author(s) -
Tang HaoNing,
Xia Yu,
Yu Yang,
Wu RuiXin,
Gao LiNa,
Chen FaMing
Publication year - 2016
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12501
Subject(s) - periodontal ligament stem cells , mesenchymal stem cell , stem cell , regeneration (biology) , periodontal fiber , periostin , periodontitis , microbiology and biotechnology , immunology , chemistry , pathology , medicine , extracellular matrix , biology , dentistry , alkaline phosphatase , biochemistry , enzyme
Abstract Aim The aim of this study was to compare the properties of stem cells derived from “inflamed” and healthy periodontal ligament ( PDL ) tissues from patient‐matched groups. Material and Methods Patient‐matched stem cells derived from root‐attached “inflamed” and healthy PDL tissues from six donors, termed I‐ PDLSC s and H‐ PDLSC s, respectively, were investigated with regard to their stem cell properties, immunomodulatory effects and capacity to form robust cell sheets for therapeutic applications. Results We found that cells derived from both sources exhibited typical mesenchymal stem cell ( MSC ) characteristics. However, compared with H‐ PDLSC s, I‐ PDLSC s demonstrated an increased capacity to proliferate, a greater potential to migrate and a decreased capacity to differentiate into osteoblasts in vitro. When I‐ PDLSC s and H‐ PDLSC s were co‐cultured with peripheral blood mononuclear cells, the MSC s derived from “inflamed” PDL tissues exhibited impaired immunomodulation. Although I‐ PDLSC s led to increased collagen type I, periostin and integrin β 1 content in the matrix, the cell sheets formed by I‐ PDLSC s were dysfunctional due to their impaired osteogenic/chondrogenic differentiation and tissue regeneration. Conclusions These data provide additional evidence that I‐ PDLSC s are functionally compromised compared with H‐ PDLSC s. Nonetheless, their dominant abundance in the available tissues indicates that stem cells derived from damaged teeth extracted due to periodontitis warrant further exploration.