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Diagnostic accuracy for apical and chronic periodontitis biomarkers in gingival crevicular fluid: an exploratory study
Author(s) -
Baeza Mauricio,
Garrido Mauricio,
HernándezRíos Patricia,
Dezerega Andrea,
GarcíaSesnich Jocelyn,
Strauss Franz,
Aitken Juan Pablo,
Lesaffre Emmanuel,
Vanbelle Sophie,
Gamonal Jorge,
BrignardelloPetersen Romina,
Tervahartiala Taina,
Sorsa Timo,
Hernández Marcela
Publication year - 2016
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12479
Subject(s) - medicine , periodontitis , dentistry , chronic periodontitis , porphyromonas gingivalis
Aim The aim of this study was to assess the levels and diagnostic accuracy of a set of potential biomarkers of periodontal tissue metabolism in gingival crevicular fluid ( GCF ) from patients with chronic periodontitis ( CP ) and asymptomatic apical periodontitis ( AAP). Materials and Methods Thirty one GCF samples from 11 CP patients, 44 GCF samples from 38 AAP patients and 31 GCF samples from 13 healthy volunteers were obtained ( N = 106). Matrix metalloproteinases ( MMP s) ‐2 and ‐9 were determined by zymography; levels of MMP ‐8 by ELISA and IFMA and MPO by ELISA . IL ‐1, IL ‐6, TNF α , DKK ‐1, Osteonectin, Periostin, TRAP ‐5 and OPG were determined by a multiplex quantitative panel. Statistical analysis was performed using linear mixed‐effects models. Results The MMP ‐9 and MMP ‐8 were higher in CP , followed by AAP , versus healthy individuals ( p < 0.05). Pro MMP ‐2, MPO , IL ‐1, IL ‐6, PTN , TRAP ‐5 and OPG were significantly higher in CP when compared with AAP and healthy patients ( p < 0.05). The highest diagnostic accuracies were observed for Pro MMP ‐2, Pro MMP ‐9, MMP ‐8 and TRAP ‐5 ( AUC > 0.97) in CP , and for the active form of MMP ‐9 and MMP ‐8 ( AUC > 0.90) in AAP . Conclusion Gingival crevicular fluid composition is modified by CP and AAP . MMP ‐9 and MMP ‐8 show diagnostic potential for CP and AAP , whereas MMP ‐2 and TRAP ‐5 are useful only for CP .