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Effects of TNF ‐ α blocking on experimental periodontitis and type 2 diabetes in obese diabetic Z ucker rats
Author(s) -
Grauballe Morten Bay,
Østergaard Jakob Appel,
Schou Søren,
Flyvbjerg Allan,
Holmstrup Palle
Publication year - 2015
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12442
Subject(s) - medicine , etanercept , periodontitis , diabetic nephropathy , endocrinology , diabetes mellitus , type 2 diabetes , tumor necrosis factor alpha , inflammation
Objective Tumour necrosis factor α ( TNF ‐ α ) is considered a key signalling modulator in the pathogenesis of both periodontitis ( PD ) and type 2 diabetes mellitus ( DM 2). This study aims at elucidating the effect of TNF ‐ α blocking on the interplay between PD and DM 2. Methods Obese diabetic Zucker rats and their lean littermates were divided into five treatment groups with or without periodontitis. Anti‐ TNF ‐ α treatment was provided with Etanercept injections. Diabetic state was evaluated by oral glucose tolerance test, the homeostatic model assessment, free fatty acids and blood glucose. Systemic inflammation was assessed by measurement of interleukin ( IL )‐1 β , IL ‐6 and TNF ‐ α in plasma. Kidney complications were evaluated by real‐time rt PCR , creatinine clearance rate, urinary albumin excretion and increase in weight. PD was evaluated by registration of alveolar bone level. Results After 4 weeks the diabetic state was modified by Etanercept treatment with lower insulin levels and lower homeostatic model assessment. Furthermore, while kidney complications were reduced by Etanercept treatment, PD had no effect. PD was influenced by diabetic state, but the impact was attenuated by Etanercept treatment. Conclusion In this study anti‐ TNF ‐ α treatment improved glucose tolerance and compensated for the increased periodontal disease in obese diabetic Zucker. PD did not influence diabetic parameters assessed including complications of the rats kidneys.