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Relationship among salivary antioxidant activity, cytokines, and periodontitis: the Nagasaki Island study
Author(s) -
Tamaki Naofumi,
Yoshino Fumihiko,
Fukui Makoto,
Hayashida Hideaki,
Yoshida Ayaka,
Kitamura Masayasu,
Iwasaki Takahiro,
Furugen Reiko,
Kawasaki Koji,
Nakazato Mio,
Maeda Takahiro,
Kokeguchi Susumu,
Yamamoto Tatsuo,
Lee Masaichi Changil,
Ito HiroO,
Saito Toshiyuki
Publication year - 2015
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12438
Subject(s) - saliva , superoxide , hydroxyl radical , antioxidant , chemistry , periodontitis , chronic periodontitis , cytokine , reactive oxygen species , immunology , medicine , pharmacology , biochemistry , enzyme
Aim Antioxidant activities and cytokine levels in human body fluids are considered to be strongly associated with periodontitis. The aim of this study was to elucidate the relationship between salivary antioxidant activities against superoxide or hydroxyl radical, cytokines, and periodontal conditions through a community‐based cross‐sectional study conducted in Goto city, Japan. Materials and Methods Saliva samples were analysed for superoxide or hydroxyl radical scavenging activities and cytokine levels from 160 participants. We demonstrated that saliva contained superoxide and hydroxyl radical scavenging activities by using electron spin resonance with a spin‐trapping agent. The concentrations of eight cytokines were measured using multiplex bead assays. Results There were significant differences in salivary superoxide or hydroxyl radical scavenging activity, and the levels of Interleukin‐1 β , Interleukin‐6, and Interleukin‐8 between periodontitis classifications. Multivariate stepwise logistic regression model showed that salivary superoxide and hydroxyl radical scavenging activities were significantly associated with the classification of periodontitis. In addition, salivary superoxide scavenging activity was found to have significant association with all periodontal parameters using multiple linear regression analysis. Conclusions These findings suggest that the evaluation of salivary antioxidant activities, as assessed by electron spin resonance, are associated with periodontitis and various clinical variables in community‐dwelling participants (ClinicalTrials.gov number NCT 01742728).