Premium
Periodontal regeneration employing gingival margin‐derived stem/progenitor cells in conjunction with IL ‐1ra‐hydrogel synthetic extracellular matrix
Author(s) -
Fawzy ElSayed Karim M.,
Mekhemar Mohamed K.,
BeckBroichsitter Benedicta E.,
Bähr Telse,
Hegab Marwa,
Receveur Jan,
Heneweer Carola,
Becker Stephan T.,
Wiltfang Joerg,
Dörfer Christof E.
Publication year - 2015
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12401
Subject(s) - cementum , extracellular matrix , chemistry , progenitor cell , regeneration (biology) , transplantation , mesenchymal stem cell , stem cell , periodontal fiber , andrology , dentistry , medicine , microbiology and biotechnology , pathology , surgery , biology , dentin , biochemistry
Aim This study investigated the periodontal regenerative potential of gingival margin‐derived stem/progenitor cells ( G ‐ MSC s) in conjunction with IL ‐1ra‐releasing hyaluronic acid synthetic extracellular matrix ( HA ‐s ECM ). Materials and Methods Periodontal defects were induced at four sites in eight miniature pigs in the premolar/molar area (−4 weeks). Autologus G ‐ MSC s were isolated from the free gingival margin and magnetically sorted, using anti‐ STRO ‐1 antibodies. Colony formation and multilineage differentiation potential were tested. The G ‐ MSC s were expanded and incorporated into IL ‐1ra‐loaded/unloaded HA ‐s ECM . Within every miniature pig, four periodontal defects were randomly treated with IL ‐1ra/ G ‐ MSC s/ HA ‐s ECM (test group), G ‐ MSC s/ HA ‐s ECM (positive‐control), scaling and root planing ( SRP ; negative control‐1) or left untreated (no‐treatment group; negative control 2). Differences in clinical attachment level (Δ CAL ), probing depth (Δ PD ), gingival recession (Δ GR ), radiographic defect volume (Δ RDV ), and changes in bleeding on probing ( BOP ) between baseline and 16 weeks post‐transplantation, as well as periodontal attachment level ( PAL ), junctional epithelium length ( JE ), connective tissue adhesion ( CTA ), cementum regeneration ( CR ) and bone regeneration ( BR ) at 16 weeks post‐transplantation were evaluated. Results Isolated G ‐ MSC s showed stem/progenitor cell characteristics. IL ‐1ra loaded and unloaded G ‐ MSC s/ HA ‐s ECM showed higher Δ CAL , Δ PD , Δ GR , PAL , CR and BR as well as a lower JE compared to their negative controls and improved BOP . Conclusion G ‐ MSC s in conjunction with IL ‐1ra‐loaded/unloaded HA ‐s ECM show a significant periodontal regenerative potential.