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Functionalization of titanium implants using a modular system for binding and release of VEGF enhances bone‐implant contact in a rodent model
Author(s) -
Schliephake Henning,
Rublack Jennifer,
Förster Anne,
Schwenzer Bernd,
Reichert Judith,
Scharnweber Dieter
Publication year - 2015
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12370
Subject(s) - implant , vascular endothelial growth factor , titanium , biomedical engineering , materials science , osseointegration , dentistry , surface modification , vegf receptors , chemistry , medicine , surgery , cancer research , metallurgy
Aims To test the immobilization of vascular endothelial growth factor ( VEGF 165 ) on the surface of titanium implants using DNA oligonucleotide ( ODN ) anchor strands for the ability to enhance periimplant bone formation. Materials and Methods DNA oligonucleotides were anchored to the surface of sandblasted acid‐etched ( SAE ) titanium screw implants and were hybridized with complementary strands of ODN conjugated to rh VEGF 165 . The implants were tested against blank SAE implants and SAE implants with nano‐anchored ODN . The implants were inserted into the tibiae of 36 Sprague–Dawley rats. Primary outcome parameters were bone‐implant contact ( BIC ), amount of new bone formation and periimplant bone density ( BD ). density after 1, 4 and 13 weeks. Unit of analysis has been the individual implant. Results Implants with rh VEGF 165 hybridized to ODN anchor strands exhibited significantly increased average BIC after 1 month compared to blank implants and implants with anchored ODN strands. Conclusions It is concluded that rh VEGF 165 immobilized on the surface of titanium implants through nano‐anchored oligonucleotide strands can accelerate BIC of sandblasted and etched titanium implants to a certain extent. The radius of effect of the growth factor appears to be limited to tissue immediately adjacent to the implant surface.