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Pre‐clinical evaluation of the osteogenic potential of bone morphogenetic protein‐2 loaded onto a particulate porcine bone biomaterial
Author(s) -
Yon Jeyoung,
Lee JungSeok,
Lim HyunChang,
Kim MinSoo,
Hong JiYoun,
Choi SeongHo,
Jung UiWon
Publication year - 2015
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12329
Subject(s) - biomaterial , bone morphogenetic protein 2 , in vivo , bone morphogenetic protein , chemistry , in vitro , implant , bone healing , dentistry , sinus (botany) , biomedical engineering , pathology , anatomy , medicine , surgery , biology , biochemistry , microbiology and biotechnology , botany , gene , genus
Aim The objective of study was to determine the osteogenic potential of bone morphogenetic protein‐2 ( BMP ‐2) loaded onto a particulate porcine bone mineral ( PBM ) biomaterial using a sinus augmentation model. Methods Release kinetics of BMP ‐2/ PBM was determined in vitro. Eight rabbits received BMP ‐2/ PBM or PBM alone into contra‐lateral sinus sites. The animals were killed following a 2‐week healing interval for micro‐ CT and histometrical analysis. Results Approximately 40% of the BMP ‐2 was released from PBM over the first 3 days in vitro; release maintained at a reduced level through day 21. In vivo, total augmented implant volume did not differ significantly between treatments. However, local bone formation was enhanced in the BMP ‐2/ PBM group compared with PBM control (10.5% versus 6.6%; p = 0.03), specifically in the central aspect of the PBM implant (14.2% versus 5.5%; p < 0.01) and adjoining the Schneiderian membrane (11.9% versus 5.0%; p < 0.05). There were no significant overall differences in residual biomaterial and fibrovascular tissue. Conclusion Bone morphogenetic protein‐2 enhanced local bone formation in the rabbit maxillary sinus model following implantation using a PBM carrier.