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Mandibular reconstruction using a calcium phosphate/polyethylene glycol hydrogel carrier with BMP ‐2
Author(s) -
Gruber Rudolf M.,
Krohn Sebastian,
Mauth Corinna,
Dard Michel,
Molenberg Aart,
Lange Katharina,
Perske Christina,
Schliephake Henning
Publication year - 2014
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12264
Subject(s) - polyethylene glycol , peg ratio , bone morphogenetic protein , bone morphogenetic protein 2 , dental alveolus , ethylene glycol , chemistry , dentistry , calcium , biomedical engineering , medicine , in vitro , biochemistry , finance , organic chemistry , economics , gene
Aim To test the hypothesis that a synthetic hydroxyapatite/ β ‐tricalcium phosphate ( HA / TCP ) construct combined with polyethylene glycol ( PEG ) hydrogel including recombinant human bone morphogenetic proteins‐2 (rh BMP ‐2) enhances new bone formation compared with bone morphogenetic proteins‐2 ( BMP ‐2) delivered using the HA / TCP construct alone. Material and Methods Bilateral mandibular partial thickness 20 × 8 × 8 mm (L × W × H) alveolar defects were surgically created in the edentulated posterior mandible in 18 female minipigs. Randomized into two groups of nine animals each, the alveolar defects either received HA / TCP or HA / TCP / PEG with or without BMP ‐2 (105 μ g/defect) in contra‐lateral sites using a split‐mouth design. Primary outcome, bone density (%) within four regions of interest, was evaluated following a 4‐week healing interval when the animals were killed for histometric analysis. Results Bone morphogenetic proteins‐2 loaded onto HA / TCP constructs significantly enhanced new bone formation compared with HA / TCP controls. Adding PEG apparently obstructed BMP ‐2 induced bone formation. Conclusion Polyethylene glycol compromises the osteogenic effect of BMP ‐2.