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Involvement of the Wnt‐ β ‐catenin signalling antagonists, sclerostin and dickkopf‐related protein 1, in chronic periodontitis
Author(s) -
Napimoga Marcelo Henrique,
Nametala Cynthia,
Silva Fábio Luiz,
Miranda Tamires Szeremeske,
Bossonaro Jeruza P.,
Demasi Ana Paula Dias,
Duarte Poliana Mendes
Publication year - 2014
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12245
Subject(s) - sclerostin , dkk1 , periodontitis , medicine , chronic periodontitis , wnt signaling pathway , bone resorption , osteoblast , tumor necrosis factor alpha , periodontal fiber , endocrinology , dentistry , gene , biology , biochemistry , in vitro
Aim The regulation of Wnt‐ β ‐catenin signalling, which is crucial for osteoblast differentiation and for bone resorption, is driven by critical inhibitors such as sclerostin ( SOST ) and dickkopf‐related protein 1 ( DKK 1). As such, the aim of this study was to evaluate the involvement of SOST and DKK 1 in human chronic periodontitis. Material and Methods Gingival biopsies and serum were sampled from systemically healthy non‐periodontitis ( n = 15) and chronic periodontitis subjects ( n = 15). The mRNA and protein levels of SOST, DKK1 and TNF‐ α in periodontal tissues were measured by qPCR and by enzyme‐linked immunosorbent assay (ELISA) respectively. Serum levels of SOST, DKK1 and TNF‐ α were assessed by ELISA. Results The mRNA and protein levels of SOST, DKK1 and TNF‐ α were significantly increased in the gingival tissues of the chronic periodontitis when compared to the non‐periodontitis group ( p < 0.05). In addition, circulating levels of SOST and TNF‐ α , but not DKK1, were higher in the periodontitis group than in the non‐periodontitis group ( p < 0.05). Conclusion SOST and DKK 1 were upregulated in the periodontal tissues of chronic periodontitis subjects, suggesting a possible role of these molecules on periodontal tissues.