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Non‐invasive in vivo imaging by confocal laser scanning microscopy of gingival tissues following natural plaque deposition
Author(s) -
Eberhard Jörg,
Loewen Hendrik,
Krüger Alexander,
Donner Sabine,
Stumpp Nico,
Patzlaff Mandy,
Stachs Oliver,
Reichard Maria,
Ripken Tammo,
Heisterkamp Alexander,
Stiesch Meike
Publication year - 2014
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12227
Subject(s) - confocal laser scanning microscopy , in vivo , pathology , confocal , confocal microscopy , laser microscopy , microscopy , medicine , biomedical engineering , biology , optics , microbiology and biotechnology , physics
Aim Imaging with Confocal Laser Scanning Microscopy ( CLSM ) generates high‐resolution images and may be well suited for basic research in Periodontology and Implant Dentistry. The present study was aimed to explore the in vivo application of CLSM in experimentally induced gingivitis. Materials and Methods Ten subjects were recruited and were advised to stop any oral hygiene of the upper front teeth for 7 days. The gingival tissues were observed using a Heidelberg Retina Tomograph combined with a Rostock Cornea Module at baseline and day 7. The system used a laser of 670 nm and the contrast was given by backscattering from different tissues. Each examination created 800–1200 images that were descriptively analysed. Results After 7 days of abandoned oral hygiene, plaque scores and bleeding frequencies increased. By using CLSM images tooth hard substances, cells and plaque deposits were distinguishable. Increased epithelial cell irregularities, the apical migration of the sulcular epithelium, cellular infiltrates within the sulcus and plaque deposits were observed at day 7. Conclusions The present study showed for the first time that CLSM is suitable for in vivo imaging of the gingival sulcus and adjacent tissues.