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Matrix metalloproteinases and myeloperoxidase in gingival crevicular fluid provide site‐specific diagnostic value for chronic periodontitis
Author(s) -
Leppilahti Jussi M.,
HernándezRíos Patricia A.,
Gamonal Jorge A.,
Tervahartiala Taina,
BrignardelloPetersen Romina,
Mantyla Paivi,
Sorsa Timo,
Hernández Marcela
Publication year - 2014
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12223
Subject(s) - periodontitis , chronic periodontitis , gingivitis , medicine , myeloperoxidase , matrix metalloproteinase , gastroenterology , pathology , dentistry , inflammation
Aim To identify the diagnostic accuracy of gingival crevicular fluid ( GCF ) candidate biomarkers to discriminate periodontitis from the inflamed and healthy sites, and to compare the performance of two independent matrix metalloproteinase ( MMP )‐8 immunoassays. Materials and Methods Cross sectional study . GCF ( N = 58 sites) was collected from healthy, gingivitis and chronic periodontitis volunteers and analysed for levels of azurocidin, chemokine ligand 5, MPO , TIMP ‐1 MMP ‐13 and MMP ‐14 by ELISA or activity assays. MMP ‐8 was assayed by immunofluorometric assay ( IFMA ) and ELISA . Statistical analysis was performed using linear mixed‐effects models and Bayesian statistics in R and Stata V11. Results MMP ‐8, MPO , azurocidin and total MMP ‐13 and MMP ‐14 were higher in periodontitis compared to gingivitis and healthy sites ( p < 0.05). A very high correlation between MPO and MMP ‐8 was evident in the periodontitis group ( r = 0.95, p < 0.0001). MPO , azurocidin and total levels of MMP ‐8, MMP ‐13 and MMP ‐14 showed high diagnostic accuracy (≥0.90), but only MMP ‐8 and MPO were significantly higher in the periodontitis versus gingivitis sites. MMP ‐8 determined by IFMA correlated more strongly with periodontal status and showed higher diagnostic accuracy than ELISA . Conclusions MPO and collagenolytic MMP s are highly discriminatory biomarkers for site‐specific diagnosis of periodontitis. The comparison of two quantitative MMP ‐8 methods demonstrated IFMA to be more accurate than ELISA .