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Positive correlations between h CAP 18/ LL ‐37 and chondroitin sulphate levels in chronic periodontitis
Author(s) -
Makeudom Anupong,
Kulpawaropas Samakorn,
Montreekachon Pattanin,
Khongkhunthian Sakornrat,
Sastraruji Thanapat,
Pothacharoen Peraphan,
Kongtawelert Prachya,
Krisanaprakornkit Suttichai
Publication year - 2014
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12216
Subject(s) - periodontitis , gingivitis , chronic periodontitis , chemistry , medicine , aggressive periodontitis , gastroenterology , dentistry
Abstract Aim To measure the levels of hCAP 18/LL‐37 in gingival crevicular fluid from patients with periodontal diseases compared with healthy controls and to determine the correlation between hCAP 18/LL‐37 and chondroitin sulphate (CS) levels in patients with periodontitis. Material and Methods Gingival crevicular fluid samples from 51 patients and 25 healthy volunteers were analysed for the hCAP 18/LL‐37 levels by immunoblotting and were determined for the CS levels by the competitive enzyme‐linked immunosorbent assay. Results Tris buffer pH 9.85 was selected to recover hCAP 18/LL‐37 from Periopaper strips, in which the percentages of recovery were around 70%. The median levels of hCAP 18/LL‐37 in the aggressive and the chronic periodontitis (CP) groups were significantly greater than those in the gingivitis and the healthy groups ( p < 0.05). Significant correlations between the unprocessed 18‐ kD a fragment and CS levels ( r = 0.650; p < 0.001) and between the mature 4.6‐ kD a fragment and CS levels ( r = 0.502; p < 0.001) were observed only in the CP group. Conclusion The significant correlations between the hCAP 18/LL‐37 and the CS levels were found in CP, but not in aggressive periodontitis. The presence versus absence of such correlations may be clinically applicable to help clinicians distinguish between two distinct types of periodontitis.