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Serotype‐dependent response of human dendritic cells stimulated with Aggregatibacter actinomycetemcomitans
Author(s) -
DíazZúñiga Jaime,
Yáñez Juan Pablo,
Alvarez Carla,
MelgarRodríguez Samanta,
Hernández Marcela,
Sanz Mariano,
Vernal Rolando
Publication year - 2014
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12205
Subject(s) - aggregatibacter actinomycetemcomitans , serotype , microbiology and biotechnology , lipopolysaccharide , immunogenicity , tumor necrosis factor alpha , cytokine , biology , immunology , antigen , porphyromonas gingivalis , bacteria , genetics
Aim Different serotypes of Aggregatibacter actinomycetemcomitans have been described based on the lipopolysaccharide ( LPS )‐O‐polysaccharide antigenicity. In turn, a distinct effect of A. actinomycetemcomitans serotypes has been described on cell proliferation and pro‐inflammatory cytokine production in different human cells. This study was aimed to investigate the differential dendritic cell ( DC ) response when stimulated with different bacterial strains belonging to the most prevalent serotypes of A. actinomycetemcomitans (a–c). Materials and Methods Dendritic cells were obtained from healthy subjects and stimulated with increasing multiplicity of infection ( MOI = 10 −1 −10 2 ) of A. actinomycetemcomitans, serotypes a–c, or their lipopolysaccharide (10‐50 ng/ml). The levels for interferon ( IFN )‐γ, tumour necrosis factor ( TNF )‐α, interleukin ( IL )‐1β, IL ‐5, IL ‐6, IL ‐10, IL ‐12 and IL ‐23 were quantified by real‐time RT ‐ PCR and ELISA . Results Variable DC responses were detected when stimulated with the different strains of A. actinomycetemcomitans . DC s stimulated with A. actinomycetemcomitans strains belonging to the serotype b or their purified LPS expressed higher levels of IL ‐1β, IL ‐6, IL ‐12, IL ‐23, IFN ‐γ and TNF ‐α than DC s stimulated with the other serotypes. Conclusions Aggregatibacter actinomycetemcomitans strains belonging to the serotype b demonstrated a higher capacity to trigger Th1 and Th17‐type cytokine production on DC s. These increased potential is likely explained by a higher immunogenicity of their LPS .