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Distinct human T‐lymphocyte responses triggered by Porphyromonas gingivalis capsular serotypes
Author(s) -
Vernal Rolando,
DiazGuerra Eva,
Silva Augusto,
Sanz Mariano,
GarciaSanz Jose A.
Publication year - 2014
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12176
Subject(s) - porphyromonas gingivalis , serotype , immune system , microbiology and biotechnology , biology , immunology , t lymphocyte , bacteria , genetics
Aim Porphyromonas gingivalis can synthesize an extracellular capsule and different serotypes have been described based on capsular antigenicity. On dendritic cells ( DC s), the type of capsule present plays a role on the strength of the developed immune response. This study aimed to investigate the T‐lymphocyte responses when stimulated with autologous mature DC s exposed to different P . gingivalis K‐serotypes. Materials and Methods Naïve CD 4 + T‐lymphocytes were obtained from healthy subjects and stimulated with autologous DC s primed with increasing multiplicity of infections of the different P . gingivalis K‐serotypes. The Th1, Th2, Th17 and T‐regulatory cytokines and transcription factor levels were quantified. Results Distinct types of response were detected when T‐lymphocytes were stimulated by DC s primed with the different P . gingivalis K‐serotypes. T‐lymphocytes stimulated by K1 or K2‐primed DC s elicited higher levels of Th1 and Th17‐associated cytokines, T‐bet and RORC 2 than T‐lymphocytes stimulated with DC s primed with the other serotypes. Conversely, the serotypes K3‐K5 induced higher levels of Th2‐associated cytokines and GATA ‐3 than the others. Conclusions These results demonstrate that DC s primed with the different P . gingivalis K‐serotypes elicited distinct T‐cell responses. Strains K1 (W83) and K2 ( HG 184) induced a Th1/Th17 pattern of immune response and K3 (A7A1‐28), K4 ( ATCC ®49417™ ), and K5 ( HG 1690) a Th2 response.