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Enhanced periodontal tissue regeneration by periodontal cell implantation
Author(s) -
Yu Na,
Oortgiesen Daniel A. W.,
Bronckers Antonius L. J. J.,
Yang Fang,
Walboomers X. Frank,
Jansen John A.
Publication year - 2013
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12113
Subject(s) - periodontal fiber , regeneration (biology) , periodontium , paracrine signalling , dentistry , medicine , scaffold , pathology , microbiology and biotechnology , biomedical engineering , biology , receptor
Aim Due to a lack of regenerative potential, current treatments for periodontal defects do not always provide satisfactory clinical results. Previously, the implantation of a biomaterial scaffold‐cell construct has been suggested as a clinically achievable approach. In this study, it was aimed to investigate the contribution of implanted periodontal ligament ( PDL ) cells to periodontal tissue regeneration. Materials & Methods Gelatin sponges were seeded with green fluorescent protein ( GFP ) transfected PDL or gingival fibroblasts ( GF ) cells, and implanted into a surgically created rat intrabony periodontal defect model. After six weeks, decalcified maxillae were used for histomorphometrical and immunohistochemical analyses. Results After six weeks, animals that had received the PDL cells exhibited significantly more functional bone and ligament. Furthermore, there were remarkable differences in the distribution of the transplanted cells. Periodontal ligament cells were always located directly lining the newly regenerated areas. In contrast, GF cells dispersed over the whole defect area, and did not provide a favourable effect on the regeneration of the periodontal tissues. Conclusion We concluded that PDL cells transplanted into a periodontal defect survive and favour regeneration of periodontium, possibly in a paracrine manner.