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Mesenchymal stem cells combined with barrier domes enhance vertical bone formation
Author(s) -
ZigdonGiladi Hadar,
Lewinson Dina,
Bick Tova,
Machtei Eli E.
Publication year - 2013
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12044
Subject(s) - mesenchymal stem cell , stem cell , medicine , microbiology and biotechnology , biology , pathology
Aim To enhance vertical bone formation in a rat calvarium following combination of guided bone regeneration ( GBR ) and transplantation of bone marrow derived mesenchymal stem cells (bm MSC ). Materials and Methods Gold domes (7 mm radius, 5 mm height) were filled with 5 × 10 5 bm MSC or osteogenic transformed bm MSC (ot MSC ) that were isolated from tibia of inbred rats and mixed with β TCP . Domes filled with β TCP served as control. Rats were sacrificed after 3 months. New bone formation was analysed by histology and histomorphometry. Results In all rats hard tissue filled the space under the dome. In the lower part of the specimens the newly formed mature bone was continuous with the original calvaria, whereas the upper (distal) part of the augmented tissue contained residual scaffold surrounded by connective tissue. Histomorphometric analysis revealed that cell transplantation doubled vertical bone height: (bm MSC 4.13 ± 0.5 mm, ot MSC 4.14 ± 0.3 mm β TCP 2.29 ± 0.22 mm, p ≤ 0.001). Bone area fraction (%) was significantly increased following transplantation of ot MSC (47.2 ± 2.5%) when compared with bm MSC (37.3 ± 3.35%) and with β TCP (31.09 ± 2.7%) ( p ≤ 0.031 versus bm MSC , p ≤ 0.0004 versus control) Conclusion In a rat calvaria model transplantation of both ot MSC and bm MSC , when combined with GBR significantly enhanced bone formation.