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Mesenchymal stem cells derived from inflamed periodontal ligaments exhibit impaired immunomodulation
Author(s) -
Liu Dayong,
Xu Junji,
Liu Ousheng,
Fan Zhipeng,
Liu Yi,
Wang Fu,
Ding Gang,
Wei Fulan,
Zhang Chunmei,
Wang Songlin
Publication year - 2012
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12009
Subject(s) - periodontal fiber , periodontal ligament stem cells , stem cell , mesenchymal stem cell , periodontitis , cytokine , il 2 receptor , medicine , peripheral blood mononuclear cell , dental alveolus , flow cytometry , immunology , immune system , microbiology and biotechnology , pathology , biology , t cell , in vitro , dentistry , alkaline phosphatase , biochemistry , enzyme
Aim The purpose of this study was to investigate the immunomodulatory properties of periodontal ligament stem cells derived from inflamed periodontal ligament tissues. Material and Methods Periodontal ligament stem cells were identified and isolated from healthy or inflamed periodontal ligament tissues. Peripheral blood mononuclear cells were cocultured with inflamed or healthy periodontal ligament stem cells, and T‐lymphocyte proliferation was determined by incubation with carboxyfluorescein succinimidyl ester. T‐helper cells (Th1/Th2, Th17) and regulatory T cells were analysed by flow cytometry. Cytokine profiles in supernatants were tested with a cytometric bead array. Results Compared to healthy cells, inflamed periodontal ligament stem cells showed significantly diminished inhibition of T‐cell proliferation. In cocultures, stimulated peripheral blood mononuclear cells showed significantly less induction of CD 4+ CD 25+ FOXP 3+ regulatory T cells and IL ‐10 secretion in the presence of inflamed compared with healthy periodontal ligament stem cells. Furthermore, suppression of Th17 differentiation and IL ‐17 production by inflamed periodontal ligament stem cells was significantly lesser than by healthy cells. Conclusion This study demonstrated that inflamed periodontal ligament stem cells had markedly dysfunctional immunomodulatory properties; this may contribute to an imbalanced immune response, acceleration of osteoclastogenesis and inflammatory alveolar bone loss in periodontitis.