Open AccessSERPINA5 promotes tumour cell proliferation by modulating the PI3K / AKT / mTOR signalling pathway in gastric cancer
Author(s) -
Fan Meiyang,
Xiong Xiaofan,
Han Lin,
Zhang Lingyu,
Gao Shanfeng,
Liu Liying,
Wang Xiaofei,
Huang Chen,
Tong Dongdong,
Yang Juan,
Zhao Lingyu,
Shao Yuan
Publication year - 2022
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.17514
Subject(s) - pi3k/akt/mtor pathway , cell growth , carcinogenesis , protein kinase b , cancer , proteases , cancer research , signal transduction , cell , biology , chemistry , microbiology and biotechnology , biochemistry , genetics , enzyme
Abstract SERPINA5 belongs to the serine protease inhibitor superfamily and has been reported to be lowly expressed in a variety of malignancies. However, few report of SERPINA5 in gastric cancer has been found. The purpose of this study was to determine the role of SERPINA5 in GC and to investigate potential tumorigenic mechanisms. We performed qPCR to determine the level of SERPINA5 expression in GC. We used public databases to evaluate whether SERPINA5 could be utilized to predict overall survival and disease‐free survival in GC patients. We also knocked down the expression of SERPINA5 and evaluated its effect on cell proliferation and migration. Furthermore, we explored the signal pathways and regulatory mechanisms related to SERPINA5 functions. According to our findings, SERPINA5 was shown to exhibit high expression in GC. Notably, SERPINA5 was prognostic in GC with high expression being unfavourable. SERPINA5 was further observed to promote GC tumorigenesis by modulating GC cell proliferation ability. Mechanically, SERPINA5 could inhibit CBL to regulate the PI3K/AKT/mTOR signalling pathway, thereby promoting GC carcinogenesis progression. These results highlight the important role of SERPINA5 in GC cell proliferation and suggest that SERPINA5 could be a novel target for GC treatment and a predictor for GC prognosis.