Open AccessEffects of Scoparone on differentiation, adhesion, migration, autophagy and mineralization through the osteogenic signalling pathways
Author(s) -
Park KyungRan,
Kim Bomi,
Lee Joon Yeop,
Moon HoJin,
Kwon Il Keun,
Yun HyungMun
Publication year - 2022
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.17476
Subject(s) - runx2 , bone morphogenetic protein 2 , autophagy , chemistry , microbiology and biotechnology , alkaline phosphatase , bone morphogenetic protein , signal transduction , cell adhesion , osteoblast , biochemistry , in vitro , cell , biology , enzyme , apoptosis , gene
Scoparone (SCOP), an active and efficient coumarin compound derived from Artemisia capillaris Thunb, has been used as a traditional Chinese herbal medicine. Herein, we investigated the effects of SCOP on the osteogenic processes using MC3T3‐E1 pre‐osteoblasts in in vitro cell systems. SCOP (C 11 H 10 O 4 , > 99.17%) was purified and identified from A. capillaries . SCOP (0.1 to 100 μM concentrations) did not have cytotoxic effects in pre‐osteoblasts; however, it promoted alkaline phosphatase (ALP) staining and activity, and mineralized nodule formation under early and late osteogenic induction. SCOP elevated osteogenic signals through the bone morphogenetic protein 2 (BMP2)‐Smad1/5/8 pathway, leading to the increased expression of runt‐related transcription factor 2 (RUNX2) with its target protein, matrix metallopeptidase 13 (MMP13). SCOP also induced the non‐canonical BMP2‐MAPKs pathway, but not the Wnt3a‐β‐catenin pathway. Moreover, SCOP promoted autophagy, migration and adhesion under the osteogenic induction. Overall, the findings of this study demonstrated that SCOP has osteogenic effects associated with cell differentiation, adhesion, migration, autophagy and mineralization.