Open AccessSpecific features of ex‐obese patients significantly influence the functional cell properties of adipose‐derived stromal cells
Author(s) -
Schmitz Deborah,
Robering Jan W.,
Weisbach Volker,
Arkudas Andreas,
Ludolph Ingo,
Horch Raymund E.,
Boos Anja M.,
KengelbachWeigand Annika
Publication year - 2022
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.17471
Subject(s) - adipose tissue , cd90 , stromal cell , telomere , weight loss , stem cell , biology , body mass index , telomerase , mesenchymal stem cell , medicine , physiology , immunology , pathology , endocrinology , obesity , microbiology and biotechnology , genetics , cd34 , dna , gene
Adipose‐derived stromal cells (ADSC) are increasingly used in clinical applications due to their regenerative capabilities. However, ADSC therapies show variable results. This study analysed the effects of specific factors of ex‐obese patients on ADSC functions. ADSC were harvested from abdominal tissues ( N = 20) after massive weight loss. Patients were grouped according to age, sex, current and maximum body mass index (BMI), BMI difference, weight loss method, smoking and infection at the surgical site. ADSC surface markers, viability, migration, transmigration, sprouting, differentiation potential, cytokine secretion, telomere length and mtDNA copy number were analysed. All ADSC expressed CD73, CD90, CD105, while functional properties differed significantly among patients. A high BMI difference due to massive weight loss was negatively correlated with ADSC proliferation, migration and transmigration, while age, sex or weight loss method had a smaller effect. ADSC from female and younger donors and individuals after weight loss by increase of exercise and diet change had a higher activity. Telomere length, mtDNA copy number, differentiation potential and the secretome did not correlate with patient factors or cell function. Therefore, we suggest that factors such as age, sex, increase of exercise and especially weight loss should be considered for patient selection and planning of regenerative therapies.