Open AccessMatrine alleviates cisplatin‐induced acute kidney injury by inhibiting mitochondrial dysfunction and inflammation via SIRT3 / OPA1 pathway
Author(s) -
Yuan Lu,
Yang Jingchao,
Li Ying,
Yuan Longhui,
Liu Fei,
Yuan Yujia,
Tang Xiaochi
Publication year - 2022
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.17398
Subject(s) - sirt3 , pharmacology , oxidative stress , inflammation , cisplatin , matrine , sod2 , acute kidney injury , apoptosis , chemistry , medicine , immunology , sirtuin , acetylation , biochemistry , superoxide dismutase , chemotherapy , chromatography , gene
Cisplatin is extensively used to treat malignancies. However, its clinical use is always limited due to the serious side effects, especially the nephrotoxicity. Matrine (MAT), a tetracyclic quinolizine alkaloid found in sophora genus, exerts multiple pharmacological roles, including anti‐oxidative stress, anti‐inflammation and anti‐apoptosis, but the role of MAT on acute kidney injury (AKI) has not been evaluated. Here, we found that MAT potently inhibited cell injury induced by cisplatin in HK2 cells in vitro, which was associated with the inhibition of oxidative injury and NF‐κB‐mediated inflammation. Moreover, MAT treatment could activate the SIRT3/OPA1 axis and subsequently suppress the mitochondrial fragmentation and improve mitochondrial function. More importantly, SIRT3 knockdown suppressed the deacetylation of OPA1, which blocked the protective role of MAT on cisplatin‐induced cell injury. In vivo, MAT treatment alleviated renal dysfunction, histological damage and inflammation induced by cisplatin in mice. Furthermore, consistent with the founding in vitro, MAT also activated SIRT3‐mediated deacetylation of OPA1 and alleviated mitochondrial dysfunction in AKI mice. Our study proved that MAT protected against cisplatin‐induced AKI by synergic anti‐oxidative stress and anti‐inflammation actions via SIRT3/OPA1‐mediated improvement of mitochondrial function, suggesting that MAT may be a novel and effective strategy for AKI.