Open Access3,6'‐disinapoyl sucrose attenuates Aβ 1‐42 ‐ induced neurotoxicity in Caenorhabditis elegans by enhancing antioxidation and regulating autophagy
Author(s) -
Tang Xiaoli,
Zhao Yuming,
Liu Yanan,
Liu Yang,
Liu Yue,
Niu Fenxi,
Fang Fang
Publication year - 2022
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.17153
Subject(s) - neurotoxicity , caenorhabditis elegans , neuroprotection , autophagy , transgene , biology , neurodegeneration , microbiology and biotechnology , pathogenesis , pharmacology , biochemistry , gene , medicine , toxicity , immunology , disease , apoptosis
The aggregation of β‐amyloid (Aβ) has the neurotoxicity, which is thought to play critical role in the pathogenesis of Alzheimer's disease (AD). Inhibiting Aβ deposition and neurotoxicity has been considered as an important strategy for AD treatment. 3,6'‐Disinapoyl sucrose (DISS), one of the oligosaccharide esters derived from traditional Chinese medicine Polygalae Radix, possesses antioxidative activity, neuroprotective effect and anti‐depressive activity. This study was to explore whether DISS could attenuate the pathological changes of Aβ 1‐42 transgenic Caenorhabditis elegans ( C . elegans ). The results showed that DISS (5 and 50 μM) treatment significantly prolonged the life span, increased the number of egg‐laying, reduced paralysis rate, decreased the levels of lipofuscin and ROS and attenuated Aβ deposition in Aβ 1‐42 transgenic C . elegans . Gene analysis showed that DISS could up‐regulate the mRNA expression of sod ‐ 3 , gst ‐ 4 , daf ‐ 16 , bec ‐ 1 and lgg ‐ 1 , while down‐regulate the mRNA expression of daf ‐ 2 and daf ‐ 15 in Aβ 1‐42 transgenic C . elegans . These results suggested that DISS has the protective effect against Aβ 1‐42 ‐induced pathological damages and prolongs the life span of C . elegans , which may be related to the reduction of Aβ deposition and neurotoxicity by regulating expression of genes related to antioxidation and autophagy.