Open AccessOridonin prevents oxidative stress‐induced endothelial injury via promoting Nrf‐2 pathway in ischaemic stroke
Author(s) -
Li Lei,
Cheng ShuQi,
Guo Wei,
Cai ZhenYu,
Sun YuQin,
Huang XinXin,
Yang Jin,
Ji Juan,
Chen YaYun,
Dong YinFeng,
Cheng Hong,
Sun XiuLan
Publication year - 2021
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.16923
Subject(s) - oxidative stress , neuroinflammation , pharmacology , fyn , medicine , neuroprotection , stroke (engine) , pi3k/akt/mtor pathway , blood–brain barrier , protein kinase b , signal transduction , chemistry , inflammation , immunology , biochemistry , central nervous system , mechanical engineering , engineering , proto oncogene tyrosine protein kinase src
Oridonin, a natural diterpenoid compound extracted from a Chinese herb, has been proved to exert anti‐oxidative stress effects in various disease models. The aim of the present study was to investigate the protective effects of oridonin on oxidative stress‐induced endothelial injury in ischaemic stroke. We found oridonin repaired blood‐brain barrier (BBB) integrity presented with upregulation of tight junction proteins (TJ proteins) expression, inhibited the infiltration of periphery inflammatory cells and neuroinflammation and thereby reduced infarct volume in ischaemic stroke mice. Furthermore, our results showed that oridonin could protect against oxidative stress‐induced endothelial injury via promoting nuclear translocation of nuclear factor‐erythroid 2 related factor 2 (Nrf‐2). The specific mechanism could be the activation of AKT(Ser473)/GSK3β(Ser9)/Fyn signalling pathway. Our findings revealed the therapeutic effect and mechanism of oridonin in ischaemic stroke, which provided fundamental evidence for developing the extracted compound of Chinese herbal medicine into an innovative drug for ischaemic stroke treatment.