Open AccessMicroscale grooves regulate maturation development of hPSC‐CMs by the transient receptor potential channels (TRP channels)
Author(s) -
Liu Taoyan,
Zhang Siyao,
Huang Chenwu,
Ma Shuhong,
Bai Rui,
Li Yanan,
Chang Yun,
Hang Chenwen,
Saleem Amina,
Dong Tao,
Guo Tianwei,
Jiang Youxu,
Lu Wenjing,
Zhang Lina,
Jianwen Luo,
Jiang Hongfeng,
Lan Feng
Publication year - 2021
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.16429
Subject(s) - induced pluripotent stem cell , transient receptor potential channel , microbiology and biotechnology , mechanism (biology) , biology , myocyte , neuroscience , receptor , gene , embryonic stem cell , genetics , philosophy , epistemology
The use of human pluripotent stem cell‐derived cardiomyocytes (hPSC‐CMs) is limited in drug discovery and cardiac disease mechanism studies due to cell immaturity. Micro‐scaled grooves can promote the maturation of cardiomyocytes by aligning them in order, but the mechanism of cardiomyocytes alignment has not been studied. From the level of calcium activity, gene expression and cell morphology, we verified that the W20H5 grooves can effectively promote the maturation of cardiomyocytes. The transient receptor potential channels (TRP channels) also play an important role in the maturation and development of cardiomyocytes. These findings support the engineered hPSC‐CMs as a powerful model to study cardiac disease mechanism and partly mimic the myocardial morphological development. The important role of the TRP channels in the maturation and development of myocardium is first revealed.