Open AccessIdentification of novel dysregulated circular RNAs in early‐stage breast cancer
Author(s) -
Rao Arunagiri Kuha Deva Magendhra,
Arvinden Vittal Rangan,
Ramasamy Deepa,
Patel Krishna,
Meenakumari Balaiah,
Ramanathan Priya,
Sundersingh Shirley,
Sridevi Velusami,
Rajkumar Thangarajan,
Herceg Zdenko,
Gowda Harsha,
Mani Samson
Publication year - 2021
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.16324
Subject(s) - circular rna , breast cancer , microrna , biomarker , stage (stratigraphy) , cancer , rna , biology , cancer research , oncology , computational biology , gene , medicine , genetics , paleontology
Breast cancer is a major cause of cancer‐related death in women worldwide. Non‐coding RNAs are a potential resource to be used as an early diagnostic biomarker for breast cancer. Circular RNAs are a recently identified group of non‐coding RNA with a significant role in disease development with potential utility in diagnosis/prognosis in cancer. In this study, we identified 26 differentially expressed circular RNAs associated with early‐stage breast cancer. RNA sequencing and two circRNA detection tools (find_circ and DCC) were used to understand the circRNA expression signature in breast cancer. We identified hsa_circ_0006743 (circJMJD1C) and hsa_circ_0002496 (circAPPBP1) to be significantly up‐regulated in early‐stage breast cancer tissues. Co‐expression analysis identified four pairs of circRNA‐miRNA (hsa_circ_0023990 : hsa‐miR‐548b‐3p, hsa_circ_0016601 : hsa_miR‐1246, hsa_circ_0001946 : hsa‐miR‐1299 and hsa_circ_0000117:hsa‐miR‐502‐5p) having potential interaction. The miRNA target prediction and network analysis revealed mRNA possibly regulated by circRNAs. We have thus identified circRNAs of diagnostic implications in breast cancer and also observed circRNA‐miRNA interaction which could be involved in breast cancer development.