Open AccessPeriosteum progenitors could stimulate bone regeneration in aged murine bone defect model
Author(s) -
Xiao Han,
Wang Linfeng,
Zhang Tao,
Chen Can,
Chen Huabin,
Li Shengcan,
Hu Jianzhong,
Lu Hongbin
Publication year - 2020
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.15891
Subject(s) - mesenchymal stem cell , regeneration (biology) , progenitor cell , bone healing , stem cell , periosteum , microbiology and biotechnology , pathology , andrology , chemistry , biology , anatomy , medicine
Periosteal stem cells are critical for bone regeneration, while the numbers will decrease with age. This study focused on whether Prx1 + cell, a kind of periosteal stem cell, could stimulate bone regeneration in aged mice. Four weeks and 12 months old Prx1CreER‐GFP; Rosa26 tdTomato mice were used to reveal the degree of Prx1 + cells participating in the femoral fracture healing procedure. One week, 8 weeks, 12 and 24 months old Prx1CreER‐GFP mice were used to analyse the real‐time distribution of Prx1 + cells. Twelve months old C57BL/6 male mice (n = 96) were used to create the bone defect model and, respectively, received hydrogel, hydrogel with Prx1 − mesenchymal stem cells and hydrogel with Prx1 + cells. H&E staining, Synchrotron radiation‐microcomputed tomography and mechanical test were used to analyse the healing results. The results showed that tdTomato + cells were involved in bone regeneration, especially in young mice. At the same time, GFP + cells decreased significantly with age. The Prx1 + cells group could significantly improve bone regeneration in the murine bone defect model via directly differentiating into osteoblasts and had better osteogenic differentiation ability than Prx1 − mesenchymal stem cells. Our finding revealed that the quantity of Prx1 + cells might account for decreased bone regeneration ability in aged mice, and transplantation of Prx1 + cells could improve bone regeneration at the bone defect site.