Open AccessUp‐regulation of CDHR5 expression promotes malignant phenotype of pancreatic ductal adenocarcinoma
Author(s) -
Gao Junyi,
Wang Mengyi,
Li Tong,
Liu Qiaofei,
You Lei,
Liao Quan
Publication year - 2020
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.15856
Subject(s) - pancreatic cancer , cancer research , immunohistochemistry , carcinogenesis , tissue microarray , metastasis , gene isoform , phenotype , biology , adenocarcinoma , pathological , cancer , pancreatic ductal adenocarcinoma , cell , oncology , medicine , immunology , gene , biochemistry , genetics
CDHR5 has been reported to play key roles in carcinogenesis of various cancers, but its roles in pancreatic cancer have not been reported. The present study was designed to investigate its clinical value in pancreatic ductal adenocarcinoma (PDAC). Tissue microarray‐based immunohistochemistry was performed to analyse the correlation between CDHR5 expression and clinical and pathological features of PDAC, as well as the CDHR5 expression during tumour progression. Cell function assays were performed to investigate CDHR5’s effects on PDAC cells. Moreover, qRT‐PCR was applied to investigate the expression of CDHR5 isoforms in PDAC cells. Expression of CDHR5 was higher on the membrane of PDAC cells. This high expression level was associated with shorter overall survival of PDAC patients and was identified as an independent prognostic factor for overall survival by multivariate Cox regression analysis. In addition, expression level of CDHR5 presented an increased trend in the occurrence and progression of PDAC. Cell experiment suggested that CDHR5 could notably promote invasion and migration of PDAC cells. Moreover, analysis of CDHR5 isoforms indicated CDHR5‐L was the major isoform expressed in PDAC cell lines. CDHR5 appears to be a promising and novel prognostic factor for PDAC, and its promotion in PDAC metastasis might be ascribed to the isoform CDHR5‐L.