Open AccessEvidence of mesenchymal stromal cell adaptation to local microenvironment following subcutaneous transplantation
Author(s) -
Preda Mihai Bogdan,
Lupan AnaMihaela,
Neculachi Carmen Alexandra,
Leti Livia Ioana,
Fenyo Ioana Madalina,
Popescu Sinziana,
Rusu Evelyn Gabriela,
Marinescu Catalina Iolanda,
Simionescu Maya,
Burlacu Alexandrina
Publication year - 2020
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.15717
Subject(s) - transplantation , stromal cell , mesenchymal stem cell , angiogenesis , subcutaneous injection , cancer research , biology , cell , immunology , medicine , microbiology and biotechnology , bioinformatics , genetics
Subcutaneous transplantation of mesenchymal stromal cells (MSC) emerged as an alternative to intravenous administration because it avoids the pulmonary embolism and prolongs post‐transplantation lifetime. The goal of this study was to investigate the mechanisms by which these cells could affect remote organs. To this aim, murine bone marrow–derived MSC were subcutaneously transplanted in different anatomical regions and the survival and behaviour have been followed. The results showed that upon subcutaneous transplantation in mice, MSC formed multicellular aggregates and did not migrate significantly from the site of injection. Our data suggest an important role of hypoxia‐inducible signalling pathways in stimulating local angiogenesis and the ensuing modulation of the kinetics of circulating cytokines with putative protective effects at distant sites. These data expand the current understanding of cell behaviour after subcutaneous transplantation and contribute to the development of a non‐invasive cell‐based therapy for distant organ protection.