Open AccessAssociation of IFN‐γ polymorphisms with ankylosing spondylitis risk
Author(s) -
Liu Yanhui,
Zhang Guohui,
Guan Yulong,
Zhao Xiaoliang,
Wang Quan,
Li Hua,
Qi Jinhong
Publication year - 2020
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.15680
Subject(s) - odds ratio , genotype , ankylosing spondylitis , medicine , gastroenterology , confidence interval , allele , restriction fragment length polymorphism , allele frequency , genotype frequency , case control study , polymorphism (computer science) , immunology , biology , genetics , gene
The case‐control study was designed to investigate the genetic effects of interferon‐gamma ( IFN‐γ ) rs2069727 and rs1861494 polymorphisms on ankylosing spondylitis (AS) susceptibility in a Chinese Han population. Blood samples were collected from 108 AS patients and 110 healthy controls. IFN‐γ polymorphisms were genotyped by polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP). Hardy‐Weinberg equilibrium (HWE) test was performed in control group. Odds ratios (OR) with 95% confidence intervals (95% CI) were calculated using chi‐square test to evaluate the association between AS susceptibility and IFN‐γ polymorphisms, and the results were adjusted by logistic regressive analysis. The frequency of rs2069727 CC genotype was much higher in cases than that in controls, suggested its significant association with increased AS risk (adjusted OR = 5.899, 95% CI = 1.563‐22.261; P = .009). In addition, C allele also showed close association with increased risk of AS (adjusted OR = 2.052, 95% CI = 1.286‐1.704, P = 0 .003). While the genotype and allele frequencies of IFN‐γ rs1861494 polymorphism were not significantly different between patients and controls ( P > 0.05 for all), IFN‐γ rs2069727 polymorphism is significantly associated with increased AS risk in a Chinese Han Population.