Open AccessCirculating circRNA predicting the occurrence of hepatocellular carcinoma in patients with HBV infection
Author(s) -
Wu Chen,
Deng Lei,
Zhuo Han,
Chen Xiang,
Tan Zhongming,
Han Sheng,
Tang Junwei,
Qian Xiaofeng,
Yao Aihua
Publication year - 2020
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.15635
Subject(s) - hepatocellular carcinoma , circular rna , medicine , gastroenterology , chronic hepatitis , microarray , hepatectomy , carcinoma , oncology , virology , rna , biology , gene , virus , surgery , gene expression , biochemistry , resection
Abstract A microarray‐based high‐throughput screening of human circulating circular RNA (circRNA) was applied with five patients newly diagnosed with hepatocellular carcinoma (HCC), five patients with HBV‐positive chronic hepatitis (CH) and five healthy controls (NC) enrolled. The plasma of HCC patients after hepatectomy was also collected. After multiple staged validation, we obtained five circRNAs as candidate. Based on the stratified risk score analysis, three increased circRNAs including circ_0009582, circ_0037120 and circ_0140117 were confirmed as candidate circulating fingerprints for distinguishing HCC from CH or NC group. With the combination of AFP, higher sensitivity and specificity were further guaranteed, suggesting that circ_0009582, circ_0037120 and circ_0140117 may serve as potential biomarkers for predicting the occurrence of HCC in patients with HBV infection.