Open AccessLncRNA‐mRNA expression profiles and functional networks in osteoclast differentiation
Author(s) -
Liu Wenjie,
Li Zhaofeng,
Cai Zhaopeng,
Xie Zhongyu,
Li Jinteng,
Li Ming,
Cen Shuizhong,
Tang Su'an,
Zheng Guan,
Ye Guiwen,
Su Hongjun,
Wang Shan,
Wang Peng,
Shen Huiyong,
Wu Yanfeng
Publication year - 2020
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.15560
Subject(s) - osteoclast , biology , cellular differentiation , rna , microbiology and biotechnology , long non coding rna , gene expression , computational biology , genetics , gene , receptor
Human osteoclasts are differentiated from CD14 + monocytes and are responsible for bone resorption. Long non‐coding RNAs (lncRNAs) have been proved to be significantly involved in multiple biologic processes, especially in cell differentiation. However, the effect of lncRNAs in osteoclast differentiation is less appreciated. In our study, RNA sequencing (RNA‐seq) was used to identify the expression profiles of lncRNAs and mRNAs in osteoclast differentiation. The results demonstrated that expressions of 1117 lncRNAs and 296 mRNAs were significantly altered after osteoclast differentiation. qRT‐PCR assays were performed to confirm the expression profiles, and the results were almost consistent with the RNA‐seq data. GO and KEGG analyses were used to predict the functions of these differentially expressed mRNA and lncRNAs. The Path‐net analysis demonstrated that MAPK pathway, PI3K‐AKT pathway and NF‐kappa B pathway played important roles in osteoclast differentiation. Co‐expression networks and competing endogenous RNA networks indicated that ENSG257764.2‐miR‐106a‐5p‐TIMP2 may play a central role in osteoclast differentiation. Our study provides a foundation to further understand the role and underlying mechanism of lncRNAs in osteoclast differentiation, in which many of them could be potential targets for bone metabolic disease.