Open AccessCPF impedes cell cycle re‐entry of quiescent lung cancer cells through transcriptional suppression of FACT and c‐MYC
Author(s) -
Bi Ling,
Xie Chanlu,
Jiao Lijing,
Jin Shenyi,
Hnit Su Su Thae,
Mu Yao,
Wang Yilun,
Wang Qian,
Ge Guangbo,
Wang Yaqiao,
Zhao Xiaodong,
Shi Xinglong,
Kang Yani,
De Souza Paul,
Liu Tao,
Zhou Jia,
Xu Ling,
Dong Qihan
Publication year - 2020
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.14897
Subject(s) - lung cancer , cell cycle , cancer research , cancer , cancer cell , methylation , biology , cell , downregulation and upregulation , microbiology and biotechnology , messenger rna , medicine , pathology , gene , biochemistry , genetics
Blockade of cell cycle re‐entry in quiescent cancer cells is a strategy to prevent cancer progression and recurrence. We investigated the action and mode of action of CPF mixture ( C optis chinensis, P inellia ternata and F ructus trichosanthis ) in impeding a proliferative switch in quiescent lung cancer cells. The results indicated that CPF impeded cell cycle re‐entry in quiescent lung cancer cells by reduction of FACT and c‐MYC mRNA and protein levels, with concomitant decrease in H3K4 tri‐methylation and RNA polymerase II occupancy at FACT and c‐MYC promoter regions. Animals implanted with quiescent cancer cells that had been exposed to CPF had reduced tumour volume/weight. Thus, CPF suppresses proliferative switching through transcriptional suppression of FACT and the c‐MYC, providing a new insight into therapeutic target and intervention method in impeding cancer recurrence.