Open AccessApplications of sequencing technology in clinical microbial infection
Author(s) -
Yu Xiaoling,
Jiang Wenqian,
Shi Yang,
Ye Hanhui,
Lin Jun
Publication year - 2019
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.14624
Subject(s) - biology , pathogen , pathogenic bacteria , dna sequencing , infectious disease (medical specialty) , clinical diagnosis , etiology , clinical microbiology , disease , microorganism , antibiotics , microbiology and biotechnology , medicine , intensive care medicine , bacteria , pathology , genetics , dna
Infectious diseases are a type of disease caused by pathogenic microorganisms. Although the discovery of antibiotics changed the treatment of infectious diseases and reduced the mortality of bacterial infections, resistant bacterial strains have emerged. Anti‐infective therapy based on aetiological evidence is the gold standard for clinical treatment, but the time lag and low positive culture rate of traditional methods of pathogen diagnosis leads to relative difficulty in obtaining the evidence of pathogens. Compared with traditional methods of pathogenic diagnosis, next‐generation and third‐generation sequencing technologies have many advantages in the detection of pathogenic microorganisms. In this review, we mainly introduce recent progress in research on pathogenic diagnostic technology and the applications of sequencing technology in the diagnosis of pathogenic microorganisms. This review provides new insights into the application of sequencing technology in the clinical diagnosis of microorganisms.