Open AccessComprehensive proteogenomic analysis of human embryonic and induced pluripotent stem cells
Author(s) -
Parrotta Elvira Immacolata,
Scalise Stefania,
Taverna Domenico,
De Angelis Maria Teresa,
Sarro Gianmarco,
Gaspari Marco,
Santamaria Gianluca,
Cuda Giovanni
Publication year - 2019
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.14426
Subject(s) - reprogramming , embryonic stem cell , induced pluripotent stem cell , biology , stem cell , transcriptome , microbiology and biotechnology , somatic cell , computational biology , cell potency , cellular differentiation , cell type , cell , genetics , gene , gene expression
Abstract Although the concepts of somatic cell reprogramming and human‐induced pluripotent stem cells (hiPSCs) generation have undergone several analyses to validate the usefulness of these cells in research and clinic, it remains still controversial whether the hiPSCs are equivalent to human embryonic stem cells (hESCs), pointing to the need of further characterization for a more comprehensive understanding of pluripotency. Most of the experimental evidence comes from the transcriptome analysis, while a little is available on protein data, and even less is known about the post‐translational modifications. Here, we report a combined strategy of mass spectrometry and gene expression profiling for proteogenomic analysis of reprogrammed and embryonic stem cells. The data obtained through this integrated, multi‐“omics” approach indicate that a small, but still significant, number of distinct pathways is enriched in reprogrammed versus embryonic stem cells, supporting the view that pluripotency is an extremely complex, multifaceted phenomenon, with peculiarities that are characteristic of each cell type.