Open AccessCytochrome P450 26A1 modulates uterine dendritic cells in mice early pregnancy
Author(s) -
Gu AiQin,
Li DanDan,
Wei DanPing,
Liu YanQin,
Ji WenHeng,
Yang Ying,
Lin HanYan,
Peng JingPian
Publication year - 2019
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.14423
Subject(s) - cd86 , cd11c , andrology , gene knockdown , biology , immune system , bone marrow , dendritic cell , stromal cell , immunology , microbiology and biotechnology , cell culture , medicine , cancer research , t cell , phenotype , gene , biochemistry , genetics
Cytochrome P450 26A1 (CYP26A1) plays important roles in the mice peri‐implantation period. Inhibiting its expression or function leads to pregnancy failure. However, little is known about the underlying mechanisms involved, especially the relationship between CYP26A1 and immune cells. In this study, using Cyp26a1 ‐specific antisense morpholigos ( Cyp26a1 ‐MO) knockdown mice model and pCR3.1‐Cyp26a1 vaccine mice model, we found that the number of uterine CD45 + CD11c + MHCII lo‐hi F4/80 − dendritic cells (DCs) was significantly decreased in the treated mice. The percentage of mature DCs (CD86 hi ) was obviously lower and the percentage of immature DCs (CD86 lo ) was remarkably higher in uterine DCs in the treatment group than that of the control group. Further experiments found that ID2, a transcription factor associated with DCs development, and CD86, a DC mature marker molecule, were both significantly reduced in mice uteri in the treated group. In vitro, ID2 and CD86 also decreased in bone marrow‐derived DCs under Cyp26a1 ‐MO treatment. These findings provide novel information that CYP26A1 might affect the embryo implantation via modulating the differentiation and maturation of uterine DCs.