Open AccessThe effects of mechanical stretch on the biological characteristics of human adipose‐derived stem cells
Author(s) -
Fang Bin,
Liu Yanjun,
Zheng Danning,
Shan Shengzhou,
Wang Chuandong,
Gao Ya,
Wang Jing,
Xie Yun,
Zhang Yifan,
Li Qingfeng
Publication year - 2019
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.14314
Subject(s) - stem cell , mesenchymal stem cell , microbiology and biotechnology , adipose tissue , adipogenesis , regeneration (biology) , tissue engineering , chemistry , regenerative medicine , pi3k/akt/mtor pathway , biology , biomedical engineering , signal transduction , medicine , biochemistry
Adipose‐derived stem cells (ADSCs) are a subset of mesenchymal stem cells (MSCs), which have promised a vast therapeutic potential in tissue regeneration. Recent studies have demonstrated that combining stem cells with mechanical stretch may strengthen the efficacy of regenerative therapies. However, the exact influences of mechanical stretch on MSCs still remain inconclusive. In this study, human ADSCs (hADSCs) were applied cyclic stretch stimulation under an in vitro stretching model for designated duration. We found that mechanical stretch significantly promoted the proliferation, adhesion and migration of hADSCs, suppressing cellular apoptosis and increasing the production of pro‐healing cytokines. For differentiation of hADSCs, mechanical stretch inhibited adipogenesis, but enhanced osteogenesis. Long‐term stretch could promote ageing of hADSCs, but did not alter the cell size and typical immunophenotypic characteristics. Furthermore, we revealed that PI3K/AKT and MAPK pathways might participate in the effects of mechanical stretch on the biological characteristics of hADSCs. Taken together, mechanical stretch is an effective strategy for enhancing stem cell behaviour and regulating stem cell fate. The synergy between hADSCs and mechanical stretch would most likely facilitate tissue regeneration and promote the development of stem cell therapy.