Open AccessAtractylenolide II reverses the influence of lncRNA XIST/miR‐30a‐5p/ROR1 axis on chemo‐resistance of colorectal cancer cells
Author(s) -
Zhang Ruijuan,
Wang Zhijun,
Yu Qianyun,
Shen Jun,
He Wenji,
Zhou Dongqing,
Yu Qingqing,
Fan Jiawei,
Gao Shurong,
Duan Lihong
Publication year - 2019
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.14148
Subject(s) - xist , colorectal cancer , cancer research , viability assay , cancer , biology , cell growth , cell culture , long non coding rna , microrna , rna , x inactivation , genetics , gene , x chromosome
This investigation was conducted to elucidate whether atractylenolide II could reverse the role of lnc RNA XIST /miR‐30a‐5p/ ROR 1 axis in modulating chemosensitivity of colorectal cancer cells. We totally collected 294 pairs of colorectal cancer tissues and adjacent normal tissues and also purchased colorectal cancer cell lines and human embryonic kidney cell line. 5‐fluorouracil, cisplatin, mitomycin and adriamycin were designated as the chemotherapies for colorectal cell lines, and atractylenolides were arranged as the Chinese drug. The expressions of XIST , miR‐30a‐5p and ROR 1 were quantified with aid of qRT ‐ PCR or Western blot, and luciferase reporter gene assay was implemented to determine the relationships among XIST , miR‐30a‐5p and ROR 1. Our results demonstrated that XIST and ROR 1 expressions were dramatically up‐regulated, yet miR‐30a‐5p expression was down‐regulated within colorectal cancer tissues ( P < 0.05). The overexpressed XIST and ROR 1, as well as under‐expressed miR‐30a‐5p, were inclined to promote viability and proliferation of colorectal cells under the influence of chemo drugs ( P < 0.05). In addition, XIST could directly target miR‐30a‐5p, and ROR 1 acted as the targeted molecule of miR‐30a‐5p. Interestingly, atractylenolides not only switched the expressions of XIST , miR‐30a‐5p and ROR 1 within colorectal cancer cells but also significantly intensified the chemosensitivity of colorectal cancer cells ( P < 0.05). Finally, atractylenolide II was discovered to slow down the viability and proliferation of colorectal cancer cells ( P < 0.05). In conclusion, the XIST /miR‐30a‐5p/ ROR 1 axis could be deemed as pivotal markers underlying colorectal cancer, and administration of atractylenolide II might improve the chemotherapeutic efficacy for colorectal cancer.