Open AccessCTCF regulates the FoxO signaling pathway to affect the progression of prostate cancer
Author(s) -
Shan Zhengfei,
Li Yongwei,
Yu Shengqiang,
Wu Jitao,
Zhang Chengjun,
Ma Yue,
Zhuang Guimin,
Wang Jiantao,
Gao Zhenli,
Liu Dongfu
Publication year - 2019
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.14138
Subject(s) - ctcf , cell growth , microbiology and biotechnology , cancer research , hedgehog signaling pathway , signal transduction , biology , cell , apoptosis , transcription factor , gene , genetics , enhancer
The present research focuses on the influence of CCCTC ‐binding factor ( CTCF ) on prostate cancer ( PC ) via the regulation of the FoxO signalling pathway. A bioinformatics analysis was conducted to screen out target genes for CTCF in LNC aP cells and to enrich the relevant pathways in LNC aP cells. It was found that the FoxO pathway was enriched according to the Ch IP ‐seq results of CTCF . The expression of CTCF , pF oxO1a, FoxO1a, pF oxO3a and FoxO3a was tested by RT ‐ qPCR and Western blot. Inhibition of CTCF could lead to the up‐regulation of the FoxO signalling pathway. The rates of cell proliferation, cell invasion and apoptosis were examined by MTT assay, cell invasion assay and flow cytometry under different interference conditions. Down‐regulation of CTCF could suppress cell proliferation, cell invasion and facilitate cell apoptosis. Lastly, the effect of CTCF on tumour growth was determined in nude mice. Inhibition of CTCF regulated the FoxO signalling pathway, which retarded tumour growth in vivo. In conclusion, CTCF regulates the FoxO signalling pathway to affect the progress of PC .