Open AccessNeural induction of porcine‐induced pluripotent stem cells and further differentiation using glioblastoma‐cultured medium
Author(s) -
Kim Eunhye,
Kim Mirae,
Hwang SeonUng,
Kim Jongpil,
Lee Gabsang,
Park Young Seok,
Hyun SangHwan
Publication year - 2019
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.14111
Subject(s) - neurosphere , neural stem cell , induced pluripotent stem cell , nestin , microbiology and biotechnology , biology , stem cell , vimentin , in vitro , cellular differentiation , transplantation , embryonic stem cell , immunology , adult stem cell , biochemistry , immunohistochemistry , endothelial stem cell , medicine , gene
Abstract Prior to transplantation, preclinical study of safety and efficacy of neural progenitor cells (NPCs) is needed. Therefore, it is important to generate an efficient in vitro platform for neural cell differentiation in large animal models such as pigs. In this study, porcine‐induced pluripotent stem cells (iPSCs) were seeded at high cell density to a neural induction medium containing the dual Sma‐ and Mad‐related protein (SMAD) inhibitors, a TGF‐β inhibitor and BMP4 inhibitor. The dSMADi‐derived NPCs showed NPC markers such as PLAG1 , NESTIN and VIMENTIN and higher mRNA expression of Sox1 compared to the control. The mRNA expression of HOXB4 was found to significantly increase in the retinoic acid‐treated group. NPCs propagated in vitro and generated neurospheres that are capable of further differentiation in neurons and glial cells. Gliobalstoma‐cultured medium including injury‐related cytokines treated porcine iPSC‐NPCs survive well in vitro and showed more neuronal marker expression compared to standard control medium. Collectively, the present study developed an efficient method for production of neural commitment of porcine iPSCs into NPCs.