Open AccessLow‐intensity pulsed ultrasound attenuates cardiac inflammation of CVB 3‐induced viral myocarditis via regulation of caveolin‐1 and MAPK pathways
Author(s) -
Zheng Cheng,
Wu SenMin,
Lian Hao,
Lin YuanZheng,
Zhuang Rong,
Thapa Saroj,
Chen QuanZhi,
Chen YiFan,
Lin JiaFeng
Publication year - 2019
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.14098
Subject(s) - medicine , low intensity pulsed ultrasound , mapk/erk pathway , viral myocarditis , p38 mitogen activated protein kinases , inflammation , caveolin 1 , myocarditis , protein kinase a , kinase , immunology , pharmacology , therapeutic ultrasound , ultrasound , biology , radiology , microbiology and biotechnology
The aggressive immunological activity elicited by acute viral myocarditis contributes to a large amount of cardiomyocytes loss and poor prognosis of patients in clinic. Low‐intensity pulsed ultrasound ( LIPUS ), which is an effective treatment modality for osteoarthropathy, has been recently illustrated regulating the overactive inflammatory response in various diseases. Here, we aimed to investigate whether LIPUS could attenuate coxsackievirus B3 ( CVB 3) infection‐induced injury by coordinating the inflammatory response. Male BALB /c mice were inoculated intraperitoneally with CVB 3 to establish the model of acute viral myocarditis. LIPUS treatment was given on Day 1, Day 1, 3 and Day 1, 3, 5 post‐inoculation, respectively. All mice were followed up for 14 days. Day 1, 3, 5 LIPUS treatment significantly improved the survival rate, attenuated the ventricular dysfunction and ameliorated the cardiac histopathological injury of CVB 3‐infected mice. Western blotting analysis showed Day 1, 3, 5 LIPUS treatment decreased pro‐inflammatory cytokines, increased the activation of caveolin‐1 and suppressed p38 mitogen‐activated protein kinase ( MAPK ) and extracellular signal‐regulated kinase ( ERK ) signallings in heart tissue. RAW 264.7 cells were treated with lipopolysaccharides ( LPS ) to simulate the augmented inflammatory response in vivo. LIPUS treatment on RAW264.7 inhibited the expression of pro‐inflammatory cytokines, activated caveolin‐1 and suppressed p38 MAPK and ERK signallings. Transfecting RAW 264.7 with caveolin‐1 si RNA blunted the suppression of pro‐inflammatory cytokines and MAPK signallings by LIPUS treatment. Taken together, we demonstrated for the first time that LIPUS treatment attenuated the aggressive inflammatory response during acute viral myocarditis. The underlying mechanism may be activating caveolin‐1 and suppressing MAPK signallings.