Open AccessAlteration in gut microbiota caused by time‐restricted feeding alleviate hepatic ischaemia reperfusion injury in mice
Author(s) -
Ren Jinjun,
Hu Dandan,
Mao Yilei,
Yang Huayu,
Liao Wenjun,
Xu Wei,
Ge Penglei,
Zhang Hongbing,
Sang Xinting,
Lu Xin,
Zhong Shouxian
Publication year - 2019
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.14069
Subject(s) - gut flora , apoptosis , oxidative stress , reperfusion injury , ischemia , biology , liver injury , medicine , inflammation , endocrinology , gastroenterology , immunology , biochemistry
Time‐restricted feeding ( TRF ), that is, no caloric intake for 14‐16 hours each day leads to favourable nutritional outcomes. This study is the first to investigate TRF through a surgical perspective verifying its efficacy against liver ischaemia reperfusion (I/R) injury. We randomly assigned 100 10‐week‐old wild‐type male C57 BL /6 mice into two feeding regimens: TRF and ad libitum access to food. Main outcomes were evaluated at 6, 12 and 24 hours post‐I/R surgery after 12 weeks of intervention. TRF group demonstrated minor liver injury via histological study; lower serum levels of liver enzymes, glucose and lipids; higher concentrations of free fatty acid and β‐hydroxybutyrate; decreased oxidative stress and inflammatory biomarkers; as well as less severe cell apoptosis and proliferation. Further exploration indicated better gut microenvironment and intestinal epithelial tight junction function. TRF employed its positive influence on a wide spectrum of biochemical pathways and ultimately revealed protective effect against hepatic I/R injury possibly through adjusting the gut microbiota. The results referred to a strong indication of adopting better feeding pattern for surgical patients.