Open AccessEvaluation of serum exosomal Lnc RNA ‐based biomarker panel for diagnosis and recurrence prediction of bladder cancer
Author(s) -
Zhang Shujun,
Du Lutao,
Wang Lishui,
Jiang Xiumei,
Zhan Yao,
Li Juan,
Yan Keqiang,
Duan Weili,
Zhao Yinghui,
Wang Lili,
Wang Yunshan,
Shi Yuliang,
Wang Chuanxin
Publication year - 2019
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.14042
Subject(s) - bladder cancer , microvesicles , rna , receiver operating characteristic , biomarker , exosome , proportional hazards model , logistic regression , oncology , diagnostic biomarker , multivariate analysis , medicine , cancer , biology , cancer research , microrna , gene , biochemistry
Exosomes are small membrane vesicles released by many cells. These vesicles can mediate cellular communications by transmitting active molecules including long non‐coding RNA s (lnc RNAs ). In this study, our aim was to identify a panel of lnc RNA s in serum exosomes for the diagnosis and recurrence prediction of bladder cancer ( BC ). The expressions of 11 candidate lnc RNA s in exosome were investigated in training set (n = 200) and an independent validation set (n = 320) via quantitative real‐time PCR . A three‐lnc RNA panel ( PCAT ‐1, UBC 1 and SNHG 16) was finally identified by multivariate logistic regression model to provide high diagnostic accuracy for BC with an area under the receiver‐operating characteristic curve ( AUC ) of 0.857 and 0.826 in training set and validation set, respectively, which was significantly higher than that of urine cytology. The corresponding AUC s of this panel for patients with Ta, T1 and T2‐T4 were 0.760, 0.827 and 0.878, respectively. In addition, Kaplan‐Meier analysis showed that non‐muscle‐invasive BC ( NMIBC ) patients with high UBC 1 expression had significantly lower recurrence‐free survival ( P = 0.01). Multivariate Cox analysis demonstrated that UBC 1 was independently associated with tumour recurrence of NMIBC ( P = 0.018). Our study suggested that lnc RNA s in serum exosomes may serve as considerable diagnostic and prognostic biomarkers of BC .