Open AccessLong non‐coding RNA SNHG20 promotes the tumorigenesis of oral squamous cell carcinoma via targeting miR‐197/LIN28 axis
Author(s) -
Wu Jie,
Zhao Wei,
Wang Zhonghou,
Xiang Xu,
Zhang Shengchi,
Liu Lina
Publication year - 2019
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13987
Subject(s) - carcinogenesis , gene knockdown , biology , homeobox protein nanog , cancer research , rna , long non coding rna , microrna , competing endogenous rna , lin28 , microbiology and biotechnology , cell growth , cancer , cell culture , sox2 , transcription factor , genetics , gene , embryonic stem cell , induced pluripotent stem cell
Long non‐coding RNA (lnc RNA ) has been verified to participate in the tumour regulation, including oral squamous cell carcinoma ( OSCC ). Nevertheless, the role of lnc RNA SNHG 20 on OSCC still remains elusive. Here, we investigate the physiopathologic functions of lnc RNA SNHG 20 in OSCC tumorigenesis and explore its potential mechanism. Lnc RNA SNHG 20 was up‐regulated in OSCC tissue compared with adjacent non‐tumour tissue. Meanwhile, SNHG 20 was overexpressed in cancer stem‐like cells. In vitro and in vivo, loss‐of‐function experiments showed that lnc RNA SNHG 20 knockdown inhibited proliferative ability, mammosphere‐forming ability, ALDH 1 expression, stem factors ( LIN 28, Nanog, Oct4, SOX 2) and tumour growth. Bioinformatics and luciferase reporter assay revealed that miR‐197 targeted the 3′‐untranslated regions of SNHG 20 and LIN 28 by complementary binding. Validation experiments confirmed the associated functions of SNHG 20/miR‐197/ LIN 28 axis on OSCC proliferation and stemness. In summary, our results reveal the important function of SNHG 20/miR‐197/ LIN 28 axis in the oncogenesis and stemness of OSCC , suggesting the vital role of SNHG 20 in OSCC tumorigenesis.