Open AccessThe contrary functions of lnc RNA HOTAIR /miR‐17‐5p/ PTEN axis and Shenqifuzheng injection on chemosensitivity of gastric cancer cells
Author(s) -
Jia Jianguang,
Zhan Dankai,
Li Jing,
Li Zhixiang,
Li Hongbo,
Qian Jun
Publication year - 2019
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13970
Subject(s) - hotair , pten , cisplatin , cancer research , cancer , cancer cell , viability assay , cell growth , apoptosis , chemistry , biology , medicine , downregulation and upregulation , long non coding rna , chemotherapy , pi3k/akt/mtor pathway , biochemistry , gene
This study was implemented to figure out whether lnc RNA HOTAIR /miR‐17‐5p/ PTEN axis played a role that was opposite to Shenqifuzheng ( SQFZ ) injection in regulating the chemosensitivity of gastric cancer cells. The gastric cancer tissues were gathered and four gastric cancer cell lines were prepared, including BGC ‐823, MGC ‐803, SGC ‐7901, and MKN 28. Moreover, cisplatin, adriamycin, mitomycin, and 5‐fluoroura were managed as the chemo‐therapeutics, and SQFZ was prepared as a Chinese medicine. Striking distinctions of HOTAIR , miR‐17‐5p, and PTEN expressions were observed between gastric cancer tissues and para‐carcinoma normal tissues ( P < 0.05). MKN 28 was associated with the highest resistance to cisplatin, adriamycin, mitomycin, and 5‐fluoroura among all the cell types, and SQFZ significantly improved the MKN 28 cells’ sensitivity to the drugs ( P < 0.05). The over‐expressed HOTAIR and miR‐17‐5p, as well as under‐expressed PTEN tended to significantly facilitate the viability, EMT process and proliferation of MKN 28 cells that were subject to treatment of chemo‐therapies ( P < 0.05). SQFZ could amplify the effects of si‐ HOTAIR , miR‐17‐5p inhibitor, and pc DNA ‐ PTEN on boosting the chemosensitivity of gastric cancer cells ( P < 0.05). In addition, HOTAIR was also found to directly target miR‐17‐5p, and PTEN appeared to be subject to the modification of HOTAIR and miR‐17‐5p in its acting on the viability, proliferation, EMT process, and apoptosis of gastric cancer cells. The HOTAIR /miR‐17‐5p/ PTEN axis could be regarded as the potential treatment targets for gastric cancer, and adjuvant therapy of SQFZ injection could assist in further improving the treatment efficacy of chemo‐therapies for gastric cancer.