Open AccessAnalysis of changes to lncRNAs and their target mRNAs in murine jejunum after radiation treatment
Author(s) -
Lu Qianying,
Gong Wei,
Wang Jinhan,
Ji Kaihua,
Sun Xiaohui,
Xu Chang,
Du Liqing,
Wang Yan,
Liu Qiang
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13940
Subject(s) - rna , messenger rna , microbiology and biotechnology , gene expression , biology , kegg , gene , gene ontology , genetics
Lnc RNA s have been reported to play an important role in various diseases. However, their role in the radiation‐induced intestinal injury is unknown. The goal of the present study was to analyse the potential mechanistic role of lnc RNA s in the radiation‐induced intestinal injury. Mice were divided into two groups: Control (non‐irradiated) and irradiated. Irradiated mice were administered 14 Gy of abdominal irradiation ( ABI ) and were assessed 3.5 days after irradiation. Changes to the jejuna of ABI mice were analysed using RNA ‐Seq for alterations to both lnc RNA and mRNA . These results were validated using qRT ‐ PCR . Lnc RNA s targets were predicted based on analysis of lnc RNA s‐mi RNA s‐ mRNA s interaction. 29 007 lnc RNA s and 17 142 mRNA s were detected in the two groups. At 3.5 days post‐irradiation, 91 lnc RNA s and 57 lnc RNA s were significantly up‐ and downregulated respectively. Similarly, 752 mRNA s and 400 mRNA s were significantly up‐ and downregulated respectively. qRT ‐ PCR was used to verify the altered expression of four lnc RNA s ( ENSMUST 173070, AK 157361, AK 083183, AK 038898) and four mRNA s (Mboat1, Nek10, Ccl24, Cyp2c55). Gene ontology and KEGG pathway analyses indicated the predicted genes were mainly involved in the VEGF signalling pathway. This study reveals that the expression of lnc RNA s was altered in the jejuna of mice post‐irradiation. Moreover, it provides a resource for the study of lnc RNA s in the radiation‐induced intestinal injury.