Open AccessLINC 00312 represses proliferation and metastasis of colorectal cancer cells by regulation of miR‐21
Author(s) -
Li Gang,
Wang Changming,
Wang Yongbing,
Xu Bin,
Zhang Wenzhong
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13830
Subject(s) - pten , cancer research , long non coding rna , biology , gene knockdown , competing endogenous rna , colorectal cancer , cell growth , metastasis , microrna , suppressor , rna , cancer , cell culture , microbiology and biotechnology , pi3k/akt/mtor pathway , genetics , gene , signal transduction
Long non‐coding RNA s (lnc RNA s) have emerged as important regulators of cancer, including colorectal cancer ( CRC ). The exact expression pattern of long intergenic noncoding RNA 00312 ( LINC 00312) in CRC and its mechanisms of action have not been reported. Here, we found that LINC 00312 is underexpressed in CRC tissues and cell lines. Functional experiments suggested that LINC 00312 suppresses growth, migration and invasion of CRC cells in vitro and attenuates tumour proliferation and metastasis in vivo. Mechanistically, LINC 00312 was found to regulate the malignancy of CRC cells by binding to miR‐21 and by functioning as a tumour suppressor targeting PTEN . Overexpression of miR‐21 or knockdown of PTEN attenuated the LINC 00312‐mediated inhibition of CRC cell proliferation and invasion. Taken together, our results elucidate the role of the LINC 00312–miR‐21– PTEN axis in CRC cell proliferation and tumour progression and may lead to new lnc RNA ‐based diagnostics or therapeutics for CRC .