Open AccessA polymorphism rs3746444 within the pre‐miR‐499 alters the maturation of miR‐499‐5p and its antiapoptotic function
Author(s) -
Ding Wei,
Li Mengyang,
Sun Teng,
Han Di,
Guo Xiaoci,
Chen Xiao,
Wan Qinggong,
Zhang Xuejuan,
Wang Jianxun
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13813
Subject(s) - snp , single nucleotide polymorphism , myocardial infarction , microrna , rna , biology , bioinformatics , disease , gene , genetics , polymorphism (computer science) , medicine , allele , genotype
micro RNA s (mi RNA s) are non‐coding RNA s that function as post‐transcriptional regulators of cardiac development and cardiovascular diseases. Single nucleotide polymorphisms ( SNP s) in mi RNA genes are a novel class of genetic variations in the human genome that confer the risk of cardiovascular diseases. Here, we identified a polymorphism A→G (rs3746444) in miR‐499 precursor (pre‐miR‐499) that affects the maturation of miR‐499‐5p and alters its antiapoptotic function by converting stable A‐U base pair to wobble G‐U base pair in pre‐miR‐499 secondary structure. Furthermore, our results showed that the concentrations of plasma miR‐499‐5p could be correlated with myocardial infarction ( MI ) and heart failure ( HF ) patients in comparison with control subjects and polymorphism rs3746444 in miR‐499 could influence its abundance in plasma. Finally, our results also showed that the variant of polymorphism in miR‐499 influenced the severity of the myocardial infarction significantly. This is the first report to highlight the biological significance of this polymorphism on the maturation of miR‐499‐5p and its antiapoptotic role during MI . These findings may pave a way to better understand the individual variability based on mi RNA SNP s in heart diseases and may contribute to better treatment for disease severity on a personalized level.