Open AccessTRIM 32 promotes cell proliferation and invasion by activating β‐catenin signalling in gastric cancer
Author(s) -
Wang Changming,
Xu Jiapeng,
Fu Hongbing,
Zhang Yu,
Zhang Xin,
Yang Dejun,
Zhu Zhenxin,
Wei Ziran,
Hu Zunqi,
Yan Ronglin,
Cai Qingping
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13784
Subject(s) - trim , axin2 , cyclin d1 , gene knockdown , cell growth , ubiquitin ligase , cancer research , iqgap1 , biology , microbiology and biotechnology , cell cycle , ubiquitin , cell , wnt signaling pathway , signal transduction , chemistry , cell culture , genetics , gene , scaffold protein , computer science , operating system
The tripartite motif ( TRIM ) family comprises more than 70 members involved in the regulation of many cellular pathways. TRIM 32 acts as an E3 ubiquitin ligase and has been reported to participate in many human cancers. Here, we aimed to investigate the role of TRIM 32 in gastric cancer ( GC ) and the clinical implications. High expression of TRIM 32 was observed in GC tissues and cell lines, and was significantly associated with poor prognosis. Knockdown TRIM 32 expression remarkably suppressed the proliferation, migration, and invasion of GC cells in vitro and tumour growth in vivo, whereas overexpression of TRIM 32 yielded the opposite results. Western blotting and quantitative reverse‐transcription PCR ( qRT ‐ PCR ) analyses revealed that up‐regulation of TRIM 32 significantly enhanced expression of β‐catenin protein and of its downstream targets TCF 1, cyclin D1, Axin2 and MMP 7 mRNA s. Moreover, we found that the mechanism behind the TRIM 32‐promoted GC progression was related to the β‐catenin signalling pathway. Collectively, these data suggest that TRIM 32 promotes GC cell proliferation, migration, and invasion by activating the β‐catenin signalling pathway.